{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T18:36:17Z","timestamp":1781375777173,"version":"3.54.1"},"reference-count":16,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2015,2,9]],"date-time":"2015-02-09T00:00:00Z","timestamp":1423440000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this research, super-paramagnetic Fe3O4 nanoparticles (magnetic particles) were coated with Staphylococcus protein A (SPA) and coupled with polyclonal antibody (pcAb) to construct magnetic capturing probes, and HRP-conjugated phage antibody was then used as specific detecting probe to design a labeled immunosensor for trace detection of Staphylococcus aureus enterotoxin B (SEB). The linear detection range of the sensor was 0.008~125 \u00b5g\/L, the regression equation was Y = 0.487X + 1.2 (R = 0.996, N = 15,  p &lt; 0.0001), the limit of detection (LOD) was 0.008 \u00b5g\/L, and the limit of quantification (LOQ) was 0.008 \u00b5g\/L. HRP-conjugated phage antibody, SPA and magnetic particles can enhance the sensitivity 4-fold, 3-fold and 2.6-fold higher, respectively. Compared with conventional double-antibody sandwich ELISA, the detection sensitivity of the sensor was 31-fold higher resulting from the integrated amplifying effect. The immunosensor  integrates the unique advantages of SPA-oriented antibody as magnetic capturing probe, HRP-conjugated phage antibody as detecting probe, magnetic separation immunoassay technique, and several other advanced techniques, so it achieves high sensitivity, specificity and interference-resistance. It is proven to be well suited for analysis of trace SEB in various environmental samples with high recovery rate and reproducibility.<\/jats:p>","DOI":"10.3390\/s150203896","type":"journal-article","created":{"date-parts":[[2015,2,9]],"date-time":"2015-02-09T10:17:04Z","timestamp":1423477024000},"page":"3896-3910","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Nano-Magnetic Immunosensor Based on Staphylococcus Protein A and the Amplification Effect of HRP-Conjugated Phage Antibody"],"prefix":"10.3390","volume":"15","author":[{"given":"Xihui","family":"Mu","sequence":"first","affiliation":[{"name":"Research Institute of Chemical Defence, State Key Laboratory of NBC Protection for Civilian,  Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Tong","sequence":"additional","affiliation":[{"name":"Research Institute of Chemical Defence, State Key Laboratory of NBC Protection for Civilian,  Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qibin","family":"Huang","sequence":"additional","affiliation":[{"name":"Research Institute of Chemical Defence, State Key Laboratory of NBC Protection for Civilian,  Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Liu","sequence":"additional","affiliation":[{"name":"Research Institute of Chemical Defence, State Key Laboratory of NBC Protection for Civilian,  Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwei","family":"Liu","sequence":"additional","affiliation":[{"name":"Research Institute of Chemical Defence, State Key Laboratory of NBC Protection for Civilian,  Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanqun","family":"Hao","sequence":"additional","affiliation":[{"name":"Research Institute of Chemical Defence, State Key Laboratory of NBC Protection for Civilian,  Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Research Institute of Chemical Defence, State Key Laboratory of NBC Protection for Civilian,  Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Research Institute of Chemical Defence, State Key Laboratory of NBC Protection for Civilian,  Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Research Institute of Chemical Defence, State Key Laboratory of NBC Protection for Civilian,  Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,2,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.aca.2011.02.031","article-title":"Enzyme-linked immunosensor based on super paramagnetic nanobeads for easy and rapid detection of okadaic acid","volume":"690","author":"Hayat","year":"2011","journal-title":"Anal. 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