Sniffles2 is an open-source software for reliable detection of structural variants (50 bp and upward) from long-read sequencing. Sniffles2 is able to call variants over a wide variant allele fraction (5–100%) and across multiple samples (population).

This is a protocol to bioprint cells within thin, flat extracellular matrix squares in 96-well plates to establish three-dimensional organoid cultures for efficient, high-throughput, time-resolved quantitative phase imaging at single-organoid resolution.

The authors propose that researchers consider nonexhaustive extraction methods—for example, solid phase microextraction—as an environmentally responsible alternative for sampling and analyte enrichment in laboratory, field and clinical studies.

This Protocol is for the identification of functional neural networks measured using resting state functional magnetic resonance imaging using an automated algorithm that can localize personalized targets for neuromodulation.

This protocol enables users to build a 3D microfluidic human bone marrow-on-a-chip microphysiological system with biomimetic tissue strutures, which enables in vitro study of human hematological malignancies and preclinical therapeutic screening.

Bis(monoacylglycero)phosphate (BMP) is an anionic phospholipid found in late endosomes and lysosomes that is dysregulated in an array of diseases. This protocol describes LC/MS analysis of BMP using critical biological control samples.

This protocol presents a method for converting fibroblasts from patients with Alzheimer’s disease (AD) into cortical neurons, enabling the investigation of the molecular events underpinning AD-associated neurodegeneration and therapeutic testing.

Concurrent positional dynamics and activity mapping (C-DAM) allows simultaneous tracking of protein location, binding dynamics and enzymatic activity on DNA substrates by integrating optical tweezers with confocal fluorescence microscopy.

Site-selective cysteine modification enables the controlled attachment of functional payloads to antibodies. This Protocol describes the synthesis of tetra-divinylpyrimidine (tetraDVP) cysteine-reactive linkers for single payload incorporation.

Sniffles2 is an open-source software for reliable detection of structural variants (50 bp and upward) from long-read sequencing. Sniffles2 is able to call variants over a wide variant allele fraction (5–100%) and across multiple samples (population).

This is a protocol to bioprint cells within thin, flat extracellular matrix squares in 96-well plates to establish three-dimensional organoid cultures for efficient, high-throughput, time-resolved quantitative phase imaging at single-organoid resolution.

Since Nature Protocols launched in 2006, protocol sharing has shifted from the margins to mainstream practice. Before the launch of online protocol journals, researchers relied on protocol textbooks, research papers and word of mouth. Now, researchers can choose from a range of platforms and journals to access and disseminate detailed step-by-step protocols.

The authors propose that researchers consider nonexhaustive extraction methods—for example, solid phase microextraction—as an environmentally responsible alternative for sampling and analyte enrichment in laboratory, field and clinical studies.