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. 1994 Dec 15;205(2):1399-408.
doi: 10.1006/bbrc.1994.2821.

Molecular cloning and sequence analysis of a human 372-kDA protein localized in the Golgi complex

M Sohda  1 , Y Misumi, T Fujiwara, M Nishioka, Y Ikehara
Affiliations

Molecular cloning and sequence analysis of a human 372-kDA protein localized in the Golgi complex

M Sohda et al. Biochem Biophys Res Commun. .

Abstract

Autoantibodies from a patient with chronic rheumatoid arthritis recognized an antigen localized in the Golgi complex of various cells from different tissues and species. The autoantibodies were used as a probe for screening human QGP-1 cDNA library, resulting in identification of a 10.3-kb cDNA. The cDNA insert contained an open reading frame which encodes a 3225-residue protein with a calculated mass of 372 kDa. The predicted protein was found to have no NH2-terminal signal sequence but a single hydrophobic domain at the COOH terminus. These results indicate that the 372-kDa antigen is cytoplasmically disposed and anchored to the Golgi membrane by the COOH-terminal hydrophobic domain.

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