Biochemical and molecular characterization of the isocitrate dehydrogenase with dual coenzyme specificity from the obligate methylotroph Methylobacillus Flagellatus
Fig 3
Effects of pH and temperature on the activity of MfIDH.
(a) The effects of pH on the NAD+-dependent (●) and NADP+-dependent (○) activities of MfIDH from pH 5.0 to 10.0 in the presence of Mn2+. (b) The effects of temperature on NAD+-dependent (●) and NADP+-dependent (○) activities of MfIDH from 45 to 65°C. (c) Heat-inactivation profiles of NAD+-dependent (●) and NADP+-dependent (○) activities of MfIDH incubated at 50 to 80°C. The incubation time is 60 min.
